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MeCN was removed and samples were loaded into 3cc Oasis WAX extraction cartridges (Waters). After washing with water containing 1 AcOH and successively with absolute MeCN, the ABA was eluted using 80 MeCN containing 1 AcOH. MeCN was removed with nitrogen gas, and the acidified water extracts were loaded into 1cc Oasis HLB cartridges. After washing with water containing 1 AcOH, plant ABA was eluted with 80 MeCN containing 1 AcOH.
MeCN was removed, and samples were loaded into 1cc Bond Elute DEA cartridges (Agilent).